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    Razani lab

    DETAILED PROTOCOLS (routinely used by Razani Lab)

    Peritoneal Macrophage isolation
    Bone Marrow Derived Macrophage isolation

    CURRENTLY 

    UNDER 
    CONSTRUCTION

    Bone Marrow Transplantation of mice
    Mouse Embryonic Fibroblast isolation
    Adipocyte Differentiation of MEFs
    Tissue Homogenization (using beads)
    Isolation of total RNA (using columns)
    Lentiviral Transduction
    Adipocyte Differentiation of Stromal Vascular Fraction
    Serum Triglyceride assay
    Serum Cholesterol assay
    Serum Glucose assay
    Serum Free Fatty Acid assay
    Quantitative PCR
    Western blotting
    Lysosomal acid lipase (LAL) activity assay
    TUNEL assay (cell culture)
    Glucose Tolerance test (GTT)
    Insulin Tolerance test (ITT)
    Metabolic Chamber analysis (Phenomaster)
    Body Composition Analysis (EchoMRI)
    Aortic Root (frozen sectioning)

    The Science & 

    Mathematics University

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