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Razani lab
DETAILED PROTOCOLS
(routinely used by Razani Lab)
Peritoneal Macrophage isolation
Bone Marrow Derived Macrophage isolation
CURRENTLY
UNDER
CONSTRUCTION
Bone Marrow Transplantation of mice
Mouse Embryonic Fibroblast isolation
Adipocyte Differentiation of MEFs
Tissue Homogenization (using beads)
Isolation of total RNA (using columns)
Lentiviral Transduction
Adipocyte Differentiation of Stromal Vascular Fraction
Serum Triglyceride assay
Serum Cholesterol assay
Serum Glucose assay
Serum Free Fatty Acid assay
Quantitative PCR
Western blotting
Lysosomal acid lipase (LAL) activity assay
TUNEL assay (cell culture)
Glucose Tolerance test (GTT)
Insulin Tolerance test (ITT)
Metabolic Chamber analysis (Phenomaster)
Body Composition Analysis (EchoMRI)
Aortic Root (frozen sectioning)
