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DETAILED PROTOCOLS
(routinely used by Razani Lab)
Peritoneal Macrophage isolation
Bone Marrow Derived Macrophage isolation
Bone Marrow Transplantation of mice
Mouse Embryonic Fibroblast isolation
Adipocyte Differentiation of MEFs
Adipocyte Differentiation of Stromal Vascular Fraction
CURRENTLY
UNDER
CONSTRUCTION
Train
Bus
Bike
mTOR
Serum Triglyceride assay
Tissue Homogenization (using beads)
Isolation of total RNA (using columns)
Serum Cholesterol assay
Lentiviral Transduction
Serum Glucose assay
Quantitative PCR
Western blotting
Lysosomal acid lipase (LAL) activity assay
Serum Free Fatty Acid assay
TUNEL assay (cell culture)
Glucose Tolerance test (GTT)
Insulin Tolerance test (ITT)
Metabolic Chamber analysis (Phenomaster)
Body Composition Analysis (EchoMRI)
Aortic Root (frozen sectioning)
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